1) SpectraMax Glo Steady Luc Reporter Assay (6. Microplate Readers Tecan Infinite M200 Plate Reader. INTRODUCTION: Proper physiological function relies on the vascular system to distribute oxygenating blood to all tissues, return deoxygenated blood to the lungs, and maintain tissue homeostasis, including functions such as hemostasis, lipid transport, and immune surveillance. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Choose from a three- (M3), four- (M4), orThe SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. StakMax Microplate Handling Stacker. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The entire SpectraMax M range includes a cuvette port for Absorbance, Fluorescence, and from SpectraMax M3 also for Luminescence. 2. The sample was subsequently washed five times with 200 mL PBS. 10. • Tel. The instrument uses a paired monochromator and xenon flash lamp for absorbance reads allowing for optimization of wavelengths between 200 nm and 1000 nm. Place the microplate on or remove it from the microplate drawer. 0. Plant material. 0 mm hex key. 3. 3 software. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). I used the same plate and set the PMT to be automatic. A colorimetric assay was performed using a SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA) at 450 nm. A microplate reader as• FlexStation® 3 Multi-Mode Microplate Reader• SpectraMax® i3/i3x Multi-Mode Microplate Reader • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers• SpectraMax® Paradigm® Multi-Mode Microplate Reader 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg. 1. Qualify the fluorescence performance of the SpectraMax . The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 3: The cuvette chamber. 313 reference gain check fail. Other technologies such as imaging. An assay plate containing a 15-point standard curve was read on the SpectraMax. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. After cooling to room temperature (22 °C), the mixture was transferred to 96-well plates. o For example: Ex: 485/Em 525/Cutoff 515 4. spr * SpectraMax i3(x) SpectraTest ABS2 v1. e. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate Reader Biotek Synergy H1 Hybrid Microplate Reader. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Using a SpectraMax M3 spectrophotometer from Molecular Devices, we measured the color change at 450 nm. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Concisely, digested test composite was treated with NaNO 2 (5%) and incubated for 5 min. 2. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. We would like to show you a description here but the site won’t allow us. . It does not work as expected. Mitochondrial ROS concentration was determined and compared with H 2 O 2 standards using a 96-well black plate and a spectrofluorometer (SpectraMax ® M3; Molecular Devices, San Jose, CA, United States) at 540-nm emission and 580-nm extinction (Maciel et al. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 4. Page 1 SpectraMax ® GEMINI EM Dual-Scanning Microplate Spectrofluorometer Operator’s Manual 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0088 Rev. The fluorescence of the reaction was read every 3 min (λ excitation = 485 nm, λ emission = 538 nm) for up to 90 min at constant 37 °C with the SpectraMax M3 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Description1 Introduction The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. These solutions were used to measure the activity of the parasite lactate dehydrogenase (pLDH) enzyme in the cultures. I am looking to meet/connect with people from all walks of life. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. 1 release preventing the protocols from being used for validation. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. ATP standard curves read on SpectraMax microplate readers. The positive control was treated with fresh RPMI-1640 (Lonza, Walkersville, MD). Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Products : SpectraMax M3 reader, SpectraMax M4 reader, LMax II (Obsolete), FlexStation 3 reader, SpectraMax L reader, SpectraMax M5/M5e reader How do I know if the LM-1 plate is off or on? The LM1 plate has a push button switch in a cupped-out area on one end. Neutrophils were stimulated as described in tissue-culture multi-well plates. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. Additionally, SoftMax® Pro Software automatically identifies the optimal excitation and emission wavelengths via the Spectral Optimization Wizard. 1. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Metrics used to measure sensitivity include signal-to-background ratio (S/B), signal-to-noise ratio (S/N), lower limit of detection (LLD) and Z’-factor 1. 3: The cuvette chamber. 0 out of 5Growth curves were read in a Spectramax M3 plate reader, at 37 °C with shaking at an optical density of 600 nm; reads were taken every 5 min. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. We measured the fluorescence intensity for 240 min at room temperature using a spectrophotometer (SpectraMax M3, Molecular devices) with excitation and emission wavelengths of 485 and 525 nm, respectively. Turn the instrument around so that the back of the instrument is. SpectraMax® ABS Plus. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. No accessories are needed and SoftMax® Pro. Italic and boldface type are used for emphasis. 9000-0756. 7°C (gradient heating design). Sample Fluorescent Reader Setting for the ADP Hunter™ Plus (Cat. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. For a complete list of the instruments supported by this release of the8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. Learn how you can utilize microplate readers to drive landmark discoveries. Collection in December 2013 and storage until extraction of Malaysian the A. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate ReaderMolecular Devices Spectramax M3 Multi Mode Microplate Reader; Related products. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Learn how to set up protocols, customize reports, export results, and more with this PDF document. WARNING! The instrument weighs approximately 36 pounds (16. FlexStation™ III. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Immunofluorescence ASC antibody (1:200, Abcam, ab155970, UK) was used for immunofluorescence in P. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Molecular Devices Spectramax M3 Mulitmode Microplate Reader is available from The Lab World Group. 190-1000 nm, tunable in 1 nm increments. Multi-Mode Microplate Readers. Impact of fix:Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. The Challenge. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. The following document is intended to demonstrate setup of this instrument. The cuvette must be in place when you read the plate. The SpectraMax ABS Plus reader utilizes Molecular . HistologyWe have been using a SpectraMax M3 for several years and have found it excellent, but more recently have been looking to use assays which require Time Resolved Fluorescence (TRF) operation (really. The SpectraMax M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for. 4. 3) This. NETosis Assays Using Immunostaining and Microscopy. The corrected absorbance is within 5% of the. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). To measure cell viability, 74 µl/well CyQUANT ® NF DNA binding dye was added to the cells and the cells were incubated for 60 min at 37 °C before fluorescence was measured using 485 nm excitation and 530 nm emission using a Spectramax M3 multi-mode microplate reader. SpectraMax Spectrofluorometers. 2. Cables. 1 Defect ID: FB 2570 These two validation protocols are not included in the v5. 1. SpectraMax® Microplate Readers and SoftMax® Pro Software. Male 8 pin mini Din to Female 9D. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. -Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. The staphyloxanthin intermediates present in collected supernatant were quantified by measuring at different wavelengths using multi-label reader (Spectramax M3, USA). SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Ataxia telangiectasia and Rad3-related (ATR) kinase protects genome integrity during DNA replication. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. ; The top 3 importers of Spectramax are India with 365. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. book Page 1 Wednesday, July 7, 2010 2:09 PM Plate chamber design of the SpectraMax category 1 microplate readers. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. 2. Learn more about its features, specifications, and applications in this user guide. All procedures completely complied with the manufacturer’s instructions. $ 15,999. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Furthermore, to investigate the effects of nucleotides on the interaction, the assay was repeated in the presence of 5 mM. M2. First, 4T1 cells were. Dual monochromatic, three modes detection, UV Vis Abs, Fluor, and Lum, cuvette. Find many great new & used options and get the best deals for Replacement Bulb for Molecular Devices SpectraMax M3 Flash Lamp at the best online prices at eBay! Free shipping for many products! Skip to main content. The isolates were categorized. Mohd. 1) EarlyTox Glutathione Assay (6. After 20 min incubation at 37 °C in the dark, the plates were analyzed by a SpectraMax M3 microplate reader to measure the absorbance at 405 nm. SpectraMax Microplate Readers. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. On the front panel of the instrument, press the load/eject button to move the microplate drawer outside of the instrument. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. Molecular Devices SpectraMax® series microplate readers. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Learn how to use the SpectraTest ABS2 Validation Plate, a convenient and reliable tool for verifying the performance of your absorbance microplate reader. Danaher Inc molecular devices spectramax m3 spectrophotometer Molecular Devices Spectramax M3 Spectrophotometer, supplied by Danaher Inc, used in various techniques. Add to Cart. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. No. 1. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Page 22 StakMax Microplate Handling System User Guide 3. Place the plate drawer transport lock on the end of the plate drawer. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. elasticus barks done by associated professor Dr. SpectraMax M3, M4, M5, and M5 e. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. For more information, please visit us at per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. Photometric accuracy. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. 0 out of 5 $ 11,999. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. The spectrophotometer (SpectraMax, M3, Molecular Devices, Washington DC, USA) was used to confirm the encapsulation of gallic acid inside the PLGA nanoparticles. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Speed Read Mode. After that, 100 µL of TMB solution was added as the substrate and kept for 15 min at room temperature. Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $12,125. XTplusC Texture Analyser Languages: French, English and Greek. A BCIP/NBT ALP staining kit (Beyotime Institute of Biotechnology, Shanghai, China) was utilized to perform ALP staining on the 7th day according to the manufacturer's instructions. SpectraMax i3x Multi-Mode Detection Platform. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Description 1. 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. 8. edu, 814-863-3503. The absorbance was measured at 540 nm using a SpectraMax M3 Microplate Reader (Molecular Devices). D8000C; R&D Systems, Minneapolis,. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The concentration of CTC was expressed as mg catechin equivalent per g of extract. 2. Both readers feature an 8-channel read head for fast reads and. 2. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. Category 2 and 3 microplate readers use gradient heating as well. The SpectraMax Multi-Mode Microplate Reader offers both the Cuvette. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. For SpectraMax L or LMax II, LMax II. Make sure that the microplate reader feet are completely seated in the mounting cups. Data were collected using SoftMax Pro 6. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. 0 OD for earlier models. Table of Contents. The shift in emission peak is also automaticallyThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 100 µL of MycoAlert Substrate (reconstituted in MycoAlert Buffer) was immediately added to each well and the plate incubated at room temperature for 10 minutes. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Luminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The reaction was terminated with 100 µL of dinitrosalicylic acid and placed in a boiling water bath for 5 min. Features. We would like to show you a description here but the site won’t allow us. book Page 1 Wednesday, July 7, 2010 2:09 PM FlexStation® 3 Benchtop Multi-Mode Microplate Reader User Guide 0112-0127 B June 2013 FlexStation_manual. The optical densities (OD) were determined using the SpectraMax ® Plus 384 Microplate Reader (SpectraMax M3; Molecular Devices, SanJosé, CA, USA) at 450 nm and 660 nm [31,34]. MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. 1 & 7. 1. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. The SpectraTest ABS1 Validation Plate package. 0. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell. spr SpectraMax Plus 384. When a feature or capability applies to only certain readers, this exception is noted. AquaMax® 4000. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. 00 . Multimode Reader Comparison Chart. com. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini XPS reader, SpectraMax M5/M5e reader, SpectraMax Plus 384 reader, SpectraMax. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 314 low lamp level warning. Subsequently, the PBS solutions containing the cells and the MSNs were incubated at 37°C under shaking at 220 rpm for another 4 h, after which the PBS supernatants were transferred into the wells of a 96-well plate and evaluated on a fluorescent spectrophotometer (SpectraMax M3, Molecular Devices, USA) using an. LINKS to Craig…His YouTube is @BrickArmy and his business is: is COBI’s website: set in this video is #2535 M. spr SpectraMax iD3 SpectraTest ABS2 v1. Both microplate and cuvette ports can be qualified. 1 & 7. spr SpectraMax iD5 SpectraTest ABS2 v1. After lysis of the cells, the mixture of 30 µL cell supernatant and 150 µL 30 µg/mL DTNB incubated for 25 min. Add to Cart. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Our Price: $8,635. The relative LDH activity was calculated from the slope of the graph of decreasing absorbance. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M3. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. For each extract concentration, percentage parasite viability and. 1. The TPC of the samples was calculated by plotting a standard curve with different concentrations of gallic acid (10, 30, 60, 100, and 200 mg/L). Table 1. Transcreener ® ADP. The absorbance wavelengths used were: 286 nm for 4,4′-diapophytoene; 435 nm for 4,4′-diaponeurosporene; 455 nm for 4,4′-diaponeurosporenic acid; and 462 nm for. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Figure 2-23: Bottle holder To install the bottle holder: 1. . AquaMax® 2000. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. "With this complete portfolio of multi-mode configurations, we can deliver multi-mode. The detection of intracellular ROS and mitochondrial membrane potential. SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 50 0112-0115G Item Description Wavelengthrange 200-1000nm Wavelengthselection Monochromator,tunablein1nmincrementsPage 25 SpectraMax iD3 Multi-Mode Microplate Reader Installation Guide To install the transport lock on the plate drawer: 1. Operated from within SoftMax Pro Software, the StakMax Microplate Handler can hold up to 50 plates and facilitates barcode reading. Example: [Drawer]. SpectraMax M 系列多功能微孔板读板机（酶标仪）可测定紫外光 (UV) 和可见光 光吸收、荧光、发光、荧光偏振、时间分辨荧光 (TRF) 和均相时间分辨荧光 (HTRF) 。. Wavelength Range. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics光验证板，用于验证 SpectraMax M3、M4 和 M5/M5e 多功能读板机的光 学性能表现。 SoftMax Pro 内置 160 多种预存实验方法可以直接调用，可以进行数据获取和个性化的数据分析；强大的图标绘制工具和文本编辑功能，可以方便的进行各种数 据结果的展示。Popular answers (1) Sanjay Premi. Microplate Reader Shelf. 0 mm hex key to tighten screws #2 and #3 until the lock is attached to the plate drawer. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. book Page 1 Wednesday, July 7, 2010 2:09 PM. For more detailed information and technical support of Life Technologies assays including specific conditions for assay windows between 2-3 fold, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Italic and boldface type are used for emphasis. Learn more about its features, specifications, and applications in this user. . 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideThe SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. SpectraTest ABS1 Absorbance Validation Plate. 303 unable to cal dark current. ® reader to the computer. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. -Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for If application. The SpectraMax® M4 Multi-Mode Microplate Reader dual monochromator optics allow the widest range of applications without the need to change filters and delivers single-mode performance in one multi-mode platform. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. We would like to show you a description here but the site won’t allow us. To determine growth rate, the log phase of growth. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Expanded wavelength coverage. Tested w/warranty. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email [email protected] i3x Multi-Mode Detection Platform Features User-configurable applications User-configurable detection modules expand the reader’s detection capabilities to. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. In the SoftMax Pro Software, select the Operation tab and click Change Aperture to display a message. SoftMax Pro GxP is a special software release that includes full user and permission con-SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signal A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The chamber. (2005) . 1. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The absorbance was measured at 230. Add to Cart. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. 0 out of 5 $ 2,999. Supplier: Molecular Devices. When the user applies a set temperature of 37°C, the top is heated to 37. Introduction. 00 . SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The SpectraMax M3 microplate reader (Molecular Devices, USA) was used to monitor the colour development over 5-min intervals for 30 min at 370 nm. SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3Chapter 1: Introduction The SpectraMax® Mini Multi-Mode Microplate Reader is a multi-detection instrument with a 6- well to 384-well plate read capability. Operated fromTo ensure loading of equal amounts of protein for each sample, colorimetric protein quantification was performed using a Bio-Rad Protein Assay (Bio-Rad, Hercules, CA) and a SpectraMax M3 spectrophotometer (Molecular Devices, Sunnyvale, CA). Then, 25 nM HOPS complex, 50 nM Sec18, 600 nM Sec17, and finally 100 nM Vam7 were added. SpectraMax M3, M4, M5, and M5 e. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Other technologies such as imaging. 190-1000 nm. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. The chamber. 4. e. SpectraMax® M5. With optimized reagents, option to operate the. Twenty five μL of supernatant was removed from each sample and placed in a 96-well clear bottom plate and read at 546/575 nm (excitation/emission) using a SpectraMax M3 fluorescent microplate reader (Molecular Devices, Sunnyvale, CA). Page 28 Chapter 5: Read Modes and Read Types 1. . For crystal violet. Actual shake speed is based on the microplate format. STAKMAX. All configurations. Description 1. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. Cell Degranulation. Shop For Laboratory Equipment featuring a wide variety of new, used and refurbished laboratory equipment. BioTek Synergy 2 SL Luminescence Microplate Reader with PC & Gen5 Software. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. 90-0083 series): Excitation 530 nm,. spr SpectraMax iD3 SpectraTest ABS2 v1. Choose from a three- (M3), four- (M4), orThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. 0112-0112F 3 Contents Chapter1:SpectraTestABS1ValidationPlateOverview 5 CertificateofCalibration 6 ISO-17025 6 AvailableTests 7 Chapter2:ValidationProtocols 9SpectraMax® L (software version 5. ZERO BIAS - scores, article reviews, protocol conditions and moreF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. Validate the optical performance of your absorbance . Microplate Readers include the M3, M4,.